c1 microfluidics based platform (fluidigm)
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C1 Microfluidics Based Platform, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 2081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microfluidics-based+platform/C1/pmc12422205-101-6-9
Average 96 stars, based on 2081 article reviews
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Multiplex Assay:Article Title: Cell population structure prior to bifurcation predicts efficiency of directed differentiation in human induced pluripotent cells Article Snippet: We integrated single-cell qPCR measurements with flow cytometry to connect the phenotypic states (cell surface marker profile) to the regulatory states (transcription factor expression profile). ( A ) Single cells are collected using flow cytometry at specific time points as iPSCs differentiate to cardiac progenitors cells (CPCs) as described in the methods for downstream single-cell and population analyses. ( B ) We monitor the dynamics for eight specific cell surface markers, and individual cells from distinct cell populations are sorted into wells of a 96-well plate containing a buffer optimized for single-cell cDNA synthesis. ( C ) We performed qPCR on cDNA generated from sorted single cells using Fluidigm’s microfluidics-based platform (Biomark). Article Title: Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus Article Snippet: This was done using a Article Title: LncPRESS1 is a p53-regulated lncRNA that safeguards pluripotency by disrupting SIRT6 mediated de-acetylation of histone H3K56 Article Snippet: We used a Expressing:Article Title: Cell population structure prior to bifurcation predicts efficiency of directed differentiation in human induced pluripotent cells Article Snippet: We integrated single-cell qPCR measurements with flow cytometry to connect the phenotypic states (cell surface marker profile) to the regulatory states (transcription factor expression profile). ( A ) Single cells are collected using flow cytometry at specific time points as iPSCs differentiate to cardiac progenitors cells (CPCs) as described in the methods for downstream single-cell and population analyses. ( B ) We monitor the dynamics for eight specific cell surface markers, and individual cells from distinct cell populations are sorted into wells of a 96-well plate containing a buffer optimized for single-cell cDNA synthesis. ( C ) We performed qPCR on cDNA generated from sorted single cells using Fluidigm’s microfluidics-based platform (Biomark). Article Title: Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus Article Snippet: This was done using a Article Title: LncPRESS1 is a p53-regulated lncRNA that safeguards pluripotency by disrupting SIRT6 mediated de-acetylation of histone H3K56 Article Snippet: We used a Real-time Polymerase Chain Reaction:Article Title: Cell population structure prior to bifurcation predicts efficiency of directed differentiation in human induced pluripotent cells Article Snippet: We integrated single-cell qPCR measurements with flow cytometry to connect the phenotypic states (cell surface marker profile) to the regulatory states (transcription factor expression profile). ( A ) Single cells are collected using flow cytometry at specific time points as iPSCs differentiate to cardiac progenitors cells (CPCs) as described in the methods for downstream single-cell and population analyses. ( B ) We monitor the dynamics for eight specific cell surface markers, and individual cells from distinct cell populations are sorted into wells of a 96-well plate containing a buffer optimized for single-cell cDNA synthesis. ( C ) We performed qPCR on cDNA generated from sorted single cells using Fluidigm’s microfluidics-based platform (Biomark). Article Title: Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus Article Snippet: This was done using a Article Title: LncPRESS1 is a p53-regulated lncRNA that safeguards pluripotency by disrupting SIRT6 mediated de-acetylation of histone H3K56 Article Snippet: We used a Flow Cytometry:Article Title: Cell population structure prior to bifurcation predicts efficiency of directed differentiation in human induced pluripotent cells Article Snippet: We integrated single-cell qPCR measurements with flow cytometry to connect the phenotypic states (cell surface marker profile) to the regulatory states (transcription factor expression profile). ( A ) Single cells are collected using flow cytometry at specific time points as iPSCs differentiate to cardiac progenitors cells (CPCs) as described in the methods for downstream single-cell and population analyses. ( B ) We monitor the dynamics for eight specific cell surface markers, and individual cells from distinct cell populations are sorted into wells of a 96-well plate containing a buffer optimized for single-cell cDNA synthesis. ( C ) We performed qPCR on cDNA generated from sorted single cells using Fluidigm’s microfluidics-based platform (Biomark). Article Title: Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus Article Snippet: This was done using a Article Title: LncPRESS1 is a p53-regulated lncRNA that safeguards pluripotency by disrupting SIRT6 mediated de-acetylation of histone H3K56 Article Snippet: We used a Marker:Article Title: Cell population structure prior to bifurcation predicts efficiency of directed differentiation in human induced pluripotent cells Article Snippet: We integrated single-cell qPCR measurements with flow cytometry to connect the phenotypic states (cell surface marker profile) to the regulatory states (transcription factor expression profile). ( A ) Single cells are collected using flow cytometry at specific time points as iPSCs differentiate to cardiac progenitors cells (CPCs) as described in the methods for downstream single-cell and population analyses. ( B ) We monitor the dynamics for eight specific cell surface markers, and individual cells from distinct cell populations are sorted into wells of a 96-well plate containing a buffer optimized for single-cell cDNA synthesis. ( C ) We performed qPCR on cDNA generated from sorted single cells using Fluidigm’s microfluidics-based platform (Biomark). Article Title: Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus Article Snippet: This was done using a Article Title: LncPRESS1 is a p53-regulated lncRNA that safeguards pluripotency by disrupting SIRT6 mediated de-acetylation of histone H3K56 Article Snippet: We used a cDNA Synthesis:Article Title: Cell population structure prior to bifurcation predicts efficiency of directed differentiation in human induced pluripotent cells Article Snippet: We integrated single-cell qPCR measurements with flow cytometry to connect the phenotypic states (cell surface marker profile) to the regulatory states (transcription factor expression profile). ( A ) Single cells are collected using flow cytometry at specific time points as iPSCs differentiate to cardiac progenitors cells (CPCs) as described in the methods for downstream single-cell and population analyses. ( B ) We monitor the dynamics for eight specific cell surface markers, and individual cells from distinct cell populations are sorted into wells of a 96-well plate containing a buffer optimized for single-cell cDNA synthesis. ( C ) We performed qPCR on cDNA generated from sorted single cells using Fluidigm’s microfluidics-based platform (Biomark). Article Title: Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus Article Snippet: This was done using a Article Title: LncPRESS1 is a p53-regulated lncRNA that safeguards pluripotency by disrupting SIRT6 mediated de-acetylation of histone H3K56 Article Snippet: We used a Generated:Article Title: Cell population structure prior to bifurcation predicts efficiency of directed differentiation in human induced pluripotent cells Article Snippet: We integrated single-cell qPCR measurements with flow cytometry to connect the phenotypic states (cell surface marker profile) to the regulatory states (transcription factor expression profile). ( A ) Single cells are collected using flow cytometry at specific time points as iPSCs differentiate to cardiac progenitors cells (CPCs) as described in the methods for downstream single-cell and population analyses. ( B ) We monitor the dynamics for eight specific cell surface markers, and individual cells from distinct cell populations are sorted into wells of a 96-well plate containing a buffer optimized for single-cell cDNA synthesis. ( C ) We performed qPCR on cDNA generated from sorted single cells using Fluidigm’s microfluidics-based platform (Biomark). Article Title: Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus Article Snippet: This was done using a Article Title: LncPRESS1 is a p53-regulated lncRNA that safeguards pluripotency by disrupting SIRT6 mediated de-acetylation of histone H3K56 Article Snippet: We used a |


